The painting was created by maverick motoring artist Ian Cook of Popbangcolour, who is usually seen working with radio-controlled cars.
Ian was quick to accept Nissan's challenge to work on this super-sized project using the full-size Nissan Leaf to spread strategically placed pools of glow-in-the-dark paint across the canvas with its tyres to create the image.
The painting, which took more than 61 hours to create, was painted using more than 60 litres of luminous acrylic paint, on a giant PVC-coated canvas.
The successful attempt was overseen by official Guinness World Record adjudicator Fortuna Burke and awarded the title on 20 March 2015.
Cook said: "This has been one of the most challenging paintings I've ever been asked to create but it looks fantastic!
"The full-sized glow-in-the-dark Nissan LEAF is one of the more extraordinary things that I have used as a painting tool."
The Nissan-backed painting beats the previous record of 164.6mē set by Saint Lucas, in Eindhoven, The Netherlands, on 23 January 2014.